6,6-D2-Glucose (Glucose Metabolism). These measurements are used to quantify glucose turnover and hepatic glucose production during clamps and IVGTT. A MOX-BSTFA-1%TMCS protocol is used to derivatize 6,6-d2-glucose into a volatile form, which is then separated on a GC column. The sample is then automatically injected into the mass spectrometer.
Ring-d5- and N15-phenylalanine (Protein Metabolism). These measurements allow for study of protein turnover. An acetonitrile-MTBSTFA protocol is used to derivatize ring-d5- and N15-phenylalanine into volatile forms, which then are separated on a GC column. The sample is then automatically injected into the mass spectrometer.