Fermentors: We have 4 fermentors with working volumes of 8-12 liters, 40-60 liters and 200-375 liters. All fermentors are sterilizable in place and are equipped to monitor and control pH, dissolved oxygen, temperature and foaming. All fermentors can be equipped with computer-controlled pumps for nutrient feeding.
Tissue Culture: We have a dedicated room with a 6 ft biological safety hood and 4 incubators. We have eight 3 liter spinner flasks with stir plates for suspension adapted cells as well as various T-flasks for anchorage dependent cells. We have extensive experience with HeLa S3's and adenoviral vectors in 293 cells.
![[Equipment]](http://138.26.45.7/tculture.jpg)
![[Equipment]](http://138.26.45.7/spinners.jpg)
Cell Harvesting and Protein Concentration: Two continuous-flow centrifuges (2 and 21 lpm); Three tangential flow filtration units; Two batch centrifuges; Continuous-flow french press for cell rupture; and a Lyophilizer.
Analysis and Purification: HPLC; FPLC; Two gas chromatographs; Spectrophotometer; and Gel electrophoresis.
Other: Incubator; Incubator/Shaker; Autoclave; Low-temperature freezer; Cold cabinet and walk in cold room for large scale chromotography.
Additional resources are available through the following UAB core facilities:
Molecular Biology Core
Media Prep Facility
NMR
CFAR Core Facilities
CCC Core Facilities
All UAB Core Facilities